| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176654 |
| Chromosome: | chromosome 3 |
| Location: | 6688280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197450 | (1 of 1) PTHR10799:SF676 - WINGED HELIX DNA-BINDING DOMAIN-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGGTAACTGTGTGCCTCTGACCCACC |
| Internal bar code: | TAGAACCAGCGCGGTGGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 96.134 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGACAAGGCAGCACTAC |
| Suggested primer 2: | ACTTGGGCTATTCAACACGG |