| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176668 |
| Chromosome: | chromosome 10 |
| Location: | 2979215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440700 | (1 of 1) PTHR13343:SF17 - CELLULAR REPRESSOR OF E1A-STIMULATED GENES, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAATATATTGTACAGTCAAGTACAGTCT |
| Internal bar code: | ATAGGCGATCGGCAGAGACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.2431 |
| LEAP-Seq n confirming: | 1180 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGGTACGGTGGTATGA |
| Suggested primer 2: | ACTTCCAATGTGTGTGGCAA |