Insertion junction: LMJ.RY0402.176672_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.176672
Chromosome:chromosome 3
Location:3143085
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g165050 PMA4 (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); Plasma membrane P-type calcium ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGCGCACGGCTGCTGCTGCTGCTGCT
Internal bar code:CGACGACTCGTTGGTGGACGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:290
LEAP-Seq percent confirming:46.6776
LEAP-Seq n confirming:1419
LEAP-Seq n nonconfirming:1621
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAACACAGCGCAAAGACA
Suggested primer 2:AGTCCTCAACACCGCCTATG