| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176691 |
| Chromosome: | chromosome 6 |
| Location: | 3350310 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277550 | (1 of 3) 3.5.5.1 - Nitrilase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACAGGCCGCCAGCCTCCTCCGCGTCTC |
| Internal bar code: | GCAGATAAGGGAAGAACTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 74.4444 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTCTTGGTGGAGTGGT |
| Suggested primer 2: | AAGGTGTTAGGTGTGGGGTG |