Insertion junction: LMJ.RY0402.176697_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.176697
Chromosome:chromosome 5
Location:994954
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g246650 (1 of 1) IPR023214//IPR027377 - HAD-like domain // Zinc-binding domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAAAGAAAAGTAATCCCGCAAAGAACA
Internal bar code:GTGTTGGAGTGCGCGTGAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:232
LEAP-Seq percent confirming:71.1485
LEAP-Seq n confirming:254
LEAP-Seq n nonconfirming:103
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATAGCCCCCAACTGAAACA
Suggested primer 2:GCGGAAGAAGAGTTAGGGCT