| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176726 |
| Chromosome: | chromosome 1 |
| Location: | 4068042 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026650 | (1 of 1) PTHR23293//PTHR23293:SF5 - FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE // PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGTGGGGAAGGAGCGGTGCAGGGAGG |
| Internal bar code: | ATATCGGAGTTACGTAATCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 97.7657 |
| LEAP-Seq n confirming: | 3238 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAACACGCCACCCTCAT |
| Suggested primer 2: | TATCACGACCGTGCGTTTAG |