| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176763 |
| Chromosome: | chromosome 2 |
| Location: | 6413124 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115567 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCAGAGCTGGCATTGGCTAGGGGCG |
| Internal bar code: | ATCTATCGGGGTTGACTGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 96.9412 |
| LEAP-Seq n confirming: | 412 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCAAGGAGCTGCCGTCT |
| Suggested primer 2: | TTGAATGGGGGAGTCTTGAG |