| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176787 |
| Chromosome: | chromosome 1 |
| Location: | 2747989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016250 | MLO3,MLO4 | (1 of 5) PF03094 - Mlo family (Mlo); MLO family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGTTTCCGCTGCGCGAGCAGTTCCAA |
| Internal bar code: | CAGCATAGTGAAGTTCACGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGACATTGGACCCAAAA |
| Suggested primer 2: | AGGCATCCTTGCTCATGTCT |