| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176791 |
| Chromosome: | chromosome 16 |
| Location: | 1442421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652400 | FAL18,FAP183 | (1 of 17) PF13202 - EF hand (EF-hand_5); EF-hand domain Flagellar Associated Protein 183 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAGCCGGCCCGAACGACCGCCGCCCCG |
| Internal bar code: | GCGACAATGGTCAGGAGCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACACACAGCCTACCTCG |
| Suggested primer 2: | TAACGTGTGACCAATCGCAT |