Insertion junction: LMJ.RY0402.176791_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.176791
Chromosome:chromosome_16
Location:1442485
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g652400 FAL18 Similar to Flagellar Associated Protein FAP183 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTGCGCGTGGTCAGGGAGGCCCCCGGGGG
Internal bar code:AGGCACCCCGGGGGATACTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:840
LEAP-Seq percent confirming:99.8695
LEAP-Seq n confirming:3060
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAATGCGTCGTCAAG
Suggested primer 2:TAACGTGTGACCAATCGCAT