| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176793 |
| Chromosome: | chromosome 2 |
| Location: | 4759808 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102551 | CSV1 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGGCACGAGCCGCCGTTCCCCGCCAGC |
| Internal bar code: | AAGCATGTTTAACAATCGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 99.6246 |
| LEAP-Seq n confirming: | 2919 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAATGAAGCAAGCAGTC |
| Suggested primer 2: | AACACTGCCGGGGTATAGTG |