| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176807 |
| Chromosome: | chromosome_2 |
| Location: | 6658580 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g117550 | ANK13 | Predicted protein with ankyrin repeats | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGGCTTTCTGCGCTATGCAGCTATCAGCA |
| Internal bar code: | GATCCGGGAAAATTGGGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 99.6652 |
| LEAP-Seq n confirming: | 893 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTCTAGGCCTGACTGAT |
| Suggested primer 2: | ATGTCCAACTACTCACCGGC |