Insertion junction: LMJ.RY0402.176807_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.176807
Chromosome:chromosome 2
Location:6658580
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g117550 ANK13 (1 of 1) K06694 - 26S proteasome non-ATPase regulatory subunit 10 (PSMD10); Predicted protein with ankyrin repeats 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCTTTCTGCGCTATGCAGCTATCAGCA
Internal bar code:GATCCGGGAAAATTGGGGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:99.6652
LEAP-Seq n confirming:893
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCTCTAGGCCTGACTGAT
Suggested primer 2:ATGTCCAACTACTCACCGGC