Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.176840 |
Chromosome: | chromosome_16 |
Location: | 4951956 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g685450 | DHC8 | Flagellar inner arm dynein heavy chain, dynein e (monomeric) | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTTCCACTCGCGCATCCGCGCAGCCAGCG |
Internal bar code: | CATGAGCTTTACTCAGATGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 877 |
LEAP-Seq percent confirming: | 99.59 |
LEAP-Seq n confirming: | 1943 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTGCTGGGAGTGTACCTA |
Suggested primer 2: | TCATGAGGTTCTCCACCTCC |