| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176848 |
| Chromosome: | chromosome 7 |
| Location: | 3247523 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334200 | HEL38 | (1 of 1) K13116 - ATP-dependent RNA helicase DDX41 [EC:3.6.4.13] (DDX41, ABS); DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCATTGCGTCACGTCTATATCCCGGCC |
| Internal bar code: | TTTTTTACGGACACAAGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 95.8057 |
| LEAP-Seq n confirming: | 434 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGACAGGATGTATGCGG |
| Suggested primer 2: | AAGCTCAAGTCCGAGGACAA |