| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176855 |
| Chromosome: | chromosome 12 |
| Location: | 1189431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489800 | (1 of 4) PF00887 - Acyl CoA binding protein (ACBP) | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACCGCCTGTACGCACTGCACATGCAG |
| Internal bar code: | CCAGAGTGCCATTCACACGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 98.5222 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCCATATCTTTCTCGC |
| Suggested primer 2: | CTCAACACGACACCATCCAC |