| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176857 |
| Chromosome: | chromosome 4 |
| Location: | 3576525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTCGTCGGTACCGTTACGTGGGGCCAG |
| Internal bar code: | GTGGAGGCCAGTCGTCGTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 84.3599 |
| LEAP-Seq n confirming: | 2438 |
| LEAP-Seq n nonconfirming: | 452 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTAAACCAAAGCCA |
| Suggested primer 2: | CACGCGAAGAGTAGGACACA |