| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176864 |
| Chromosome: | chromosome 4 |
| Location: | 3874303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230438 | (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTGTGTACGGCACGCTGTGGTGGGCT |
| Internal bar code: | GAGCCCCGATTGGGAGCTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 95.6035 |
| LEAP-Seq n confirming: | 7263 |
| LEAP-Seq n nonconfirming: | 334 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGGGGACCTCTTGTTAG |
| Suggested primer 2: | AAACCTCCAACTTACGGGCT |