| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176897 |
| Chromosome: | chromosome 7 |
| Location: | 5551394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351450 | (1 of 1) PTHR14030:SF4 - MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 BETA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTCCACGCGGTGGCTCGGTCCGTGTTC |
| Internal bar code: | ATTTAGAGCAGTGGTACTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.9265 |
| LEAP-Seq n confirming: | 1360 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGGACGTGGTGTTGAC |
| Suggested primer 2: | ATCGTCTGGAATGATGAGGC |