| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176903 |
| Chromosome: | chromosome 3 |
| Location: | 5578695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186700 | (1 of 7) IPR001606 - ARID DNA-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCCTTCCCATCGACGCCGGGCAGGC |
| Internal bar code: | GATGACTGGTCATGCAGGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.1579 |
| LEAP-Seq n confirming: | 471 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCAGCATATTGAAGGGC |
| Suggested primer 2: | GTCATTTGGGTTTCGCAAGT |