| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176904 |
| Chromosome: | chromosome 12 |
| Location: | 5127800 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527050 | (1 of 13) IPR001251//IPR011074 - CRAL-TRIO lipid binding domain // CRAL/TRIO, N-terminal domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCAGGATGCTGTCCACCTTGTTCTCC |
| Internal bar code: | TCCTAGTATTACGTGTGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.2 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCATCTCCGGGTAGTTGT |
| Suggested primer 2: | CCGTAACCCGAGTAGTTGGA |