| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176909 |
| Chromosome: | chromosome 7 |
| Location: | 2499005 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329500 | SUB1 | (1 of 2) 3.4.21.111 - Aqualysin 1 / Caldolysin; Secreted protease and protease inhibitor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCGTTGTTGTCGCGGCTGGCAACAATA |
| Internal bar code: | TTTTGATTCATCTCCGGTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 99.7026 |
| LEAP-Seq n confirming: | 3017 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCCACTTACATGGGGAG |
| Suggested primer 2: | CTCACACCATGTCCTCCCTT |