| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176916 |
| Chromosome: | chromosome 4 |
| Location: | 2849952 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224300 | CGL84 | (1 of 22) PTHR10994 - RETICULON; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTCGTGTGGCCAACAACATAGGTGAG |
| Internal bar code: | GTTCGGGGGATCTATGTGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATAAAGCAGGAGCACA |
| Suggested primer 2: | TTCTTTGTTCCCCCTCACAG |