| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176919 |
| Chromosome: | chromosome 10 |
| Location: | 1585355 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429350 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCCTCATCGTCCTCGTCCTCGTCGTC |
| Internal bar code: | GCGGCGCTTACTAGCACGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 85.318 |
| LEAP-Seq n confirming: | 2307 |
| LEAP-Seq n nonconfirming: | 397 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTATCTTGGAAAGCAAC |
| Suggested primer 2: | AGAAGAAGGAGAAGGCCGAG |