Insertion junction: LMJ.RY0402.176936_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.176936
Chromosome:chromosome_9
Location:1212348
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g399914 SSA8 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TCGACTGAGTCCTATGTCGGACGTGTCGGA
Internal bar code:CACCGATTTCGTCGACTGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:98.3471
LEAP-Seq n confirming:476
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGGTAGAGAAGGCATGAA
Suggested primer 2:ATAAAGGTGAAGAGCGCGAA