Insertion junction: LMJ.RY0402.176936_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.176936
Chromosome:chromosome_9
Location:1212352
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g399914 SSA8 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CAAGAACCCCTGTCCGAGAACACCTTTTTG
Internal bar code:GACCCGAGGCTTCATCGAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:99.4417
LEAP-Seq n confirming:1603
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGGTAGAGAAGGCATGAA
Suggested primer 2:ATAAAGGTGAAGAGCGCGAA