| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176945 |
| Chromosome: | chromosome 1 |
| Location: | 223824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001400 | ZMP1 | (1 of 1) 3.4.24.36 - Leishmanolysin / Promastigote surface endopeptidase; Zinc-metallopeptidase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTAGTGGACCGCTGGCCGCCAGATGAG |
| Internal bar code: | GAGGCGGGGCCAAGCAGCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.7676 |
| LEAP-Seq n confirming: | 4293 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAGGACAAGTCCAACAG |
| Suggested primer 2: | AAATGTCTTGTACCGTCGGC |