| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176953 |
| Chromosome: | chromosome 1 |
| Location: | 1047472 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006050 | LF3 | Long Flagella protein 3; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGTTATTTGCGGGTCCTGGGTGCATGGC |
| Internal bar code: | TAGCACGCATTCCGCGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 95.4919 |
| LEAP-Seq n confirming: | 3601 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCCGTGCTCAGTCTTT |
| Suggested primer 2: | GGGTCTTTAGTTCGCAGTCG |