| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176955 |
| Chromosome: | chromosome_6 |
| Location: | 5311136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g283034 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAATGGAATGGGGCAGCTGGGACATGAA |
| Internal bar code: | TACCCGCAAGGAATAAGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.4786 |
| LEAP-Seq n confirming: | 4388 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGTGGTTTGGTGATGGT |
| Suggested primer 2: | AACTCTGCGTCACAGACACG |