| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176955 |
| Chromosome: | chromosome_9 |
| Location: | 1259165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g399400 | FAP199,TGL15 | Triacylglycerol lipase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAAGCAATCAAGCACACCACACAATACCG |
| Internal bar code: | GATTCGTTCCGTTTCCGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.7338 |
| LEAP-Seq n confirming: | 4496 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATGAGGGCCACACCACT |
| Suggested primer 2: | CTCAGGAAGTGAGTAGGCCG |