| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176964 |
| Chromosome: | chromosome 13 |
| Location: | 2698658 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582150 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCCGTGGCGGCCGCCAGCCCGGCCTCC |
| Internal bar code: | TTTGTTCGCGTGGTGCCGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 84.5195 |
| LEAP-Seq n confirming: | 2102 |
| LEAP-Seq n nonconfirming: | 385 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCGCCTCCCAATAATA |
| Suggested primer 2: | CAGGTCTTCAGAATCGGAGC |