| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177024 |
| Chromosome: | chromosome 12 |
| Location: | 325676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494650 | CSV10 | Chlamydomonas-specific family protein; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGGTCGAGGGATTAAGCACGTGTTGCT |
| Internal bar code: | GGCAGTGACCCGGTTCGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.0104 |
| LEAP-Seq n confirming: | 2001 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTTACGGCAACGTGATT |
| Suggested primer 2: | ACAACCAGCCAATCAAAAGG |