| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177026 |
| Chromosome: | chromosome 4 |
| Location: | 1825405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211750 | (1 of 2) K07297 - adiponectin receptor (ADIPOR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACGATCCGTGCATGGTCTGTGTCAAT |
| Internal bar code: | GCTCCCATAGTACAGGGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 99.5973 |
| LEAP-Seq n confirming: | 6430 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTGCGCAGACGTGTTAC |
| Suggested primer 2: | GCTTGCAATTATGGGGTTGT |