| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177034 |
| Chromosome: | chromosome 3 |
| Location: | 4142395 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173450 | PAO4 | Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCGCTCGACCAGCGTGCGAAGTCGCCA |
| Internal bar code: | AAATGGGTGCTTATTTATGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.4444 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATTGAGGATGTGGGTG |
| Suggested primer 2: | TGCTTTACAGCCTGCATGTC |