Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.177070 |
Chromosome: | chromosome 5 |
Location: | 1763941 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g242502 | 3'UTR|intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTAATGTGGTCAGCCTGGTATGCCTGG |
Internal bar code: | CAATATTTCCATCTCTCATAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 756 |
LEAP-Seq percent confirming: | 99.5719 |
LEAP-Seq n confirming: | 1163 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGTAATCCGCAGTCCACC |
Suggested primer 2: | TAGCCTGTTGTGCCTCCTCT |