| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177072 |
| Chromosome: | chromosome_16 |
| Location: | 2816363 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g663200 | FAP295 | Flagellar Associated Protein, cyclic nucleotide dependent protei | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCACGGGGCGCACCTACGCGCTCAAGCGC |
| Internal bar code: | GACCCGTCTTCCCCCCCCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCAGCATCTTGACCTT |
| Suggested primer 2: | GCTAAAGCGTGAGACGTTCC |