Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.177072 |
Chromosome: | chromosome_16 |
Location: | 2816363 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g663200 | FAP295 | Flagellar Associated Protein, cyclic nucleotide dependent protei | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCACGGGGCGCACCTACGCGCTCAAGCGC |
Internal bar code: | GACCCGTCTTCCCCCCCCCCCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 710 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTGCAGCATCTTGACCTT |
Suggested primer 2: | GCTAAAGCGTGAGACGTTCC |