| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177081 |
| Chromosome: | chromosome 5 |
| Location: | 789510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248250 | (1 of 1) 1.4.1.24 - 3-dehydroquinate synthase II / DHQ synthase II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGACAGACGAAGACTTTATGCTGCCC |
| Internal bar code: | GGGGGCCGTGTAGCGCAATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 95.3347 |
| LEAP-Seq n confirming: | 940 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTGATGCTTTGGCACTC |
| Suggested primer 2: | TACTGTTGCTGCTACCGACG |