| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177097 |
| Chromosome: | chromosome 4 |
| Location: | 1993161 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218700 | EIF1,INFA | Chloroplast translation initiation factor, IF-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTTATTGCTGACAAGTTTGCAAAGTT |
| Internal bar code: | TGCCGTGGGGCCTCCCCACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1155 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGCTTACAAAGAAAGCG |
| Suggested primer 2: | GTACGGGGATGGATTCAATG |