| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177123 |
| Chromosome: | chromosome 12 |
| Location: | 2739588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAACCCGTTCACCAACATCACAGGACC |
| Internal bar code: | TGACTCCGAGGTTGCACGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1034 |
| LEAP-Seq percent confirming: | 90.677 |
| LEAP-Seq n confirming: | 1634 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCCTGCGTGTAAGCAA |
| Suggested primer 2: | TACCTGCGTAATGTCGCTGA |