Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.177127 |
Chromosome: | chromosome_2 |
Location: | 8400904 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g141606 | DHC9 | Flagellar inner arm dynein heavy chain, dynein c (monomeric) | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGGTTTGAGTGGTGGTGGGAGTGATGGT |
Internal bar code: | CGATAGTTTCAAAGAGTTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 173 |
LEAP-Seq percent confirming: | 99.3464 |
LEAP-Seq n confirming: | 152 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAAATCCACAAACCATCGCA |
Suggested primer 2: | GCTGCTCAACTTCATGACCA |