| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177145 |
| Chromosome: | chromosome 6 |
| Location: | 5719789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286850 | (1 of 1) 1.1.1.193 - 5-amino-6-(5-phosphoribosylamino)uracil reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAAGTTCAGCGATCCGGAGCAGCCTGG |
| Internal bar code: | TTTCGGGTGCCTATGGGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.8337 |
| LEAP-Seq n confirming: | 2401 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAATGTGGCCTACCAGC |
| Suggested primer 2: | TGGGAGGGATTATCAGTTCG |