| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177147 |
| Chromosome: | chromosome 7 |
| Location: | 2800782 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331650 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAAAGACCCACTTGCACAGACCGCCTT |
| Internal bar code: | GAGGGGGCGTAGCGGCAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 96.3358 |
| LEAP-Seq n confirming: | 3707 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATGCCAGTAACCCAGT |
| Suggested primer 2: | CCTGCCTTAAAGAGCTGGTG |