| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177157 |
| Chromosome: | chromosome 12 |
| Location: | 7603441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555050 | MUS81,CJE1 | (1 of 1) PTHR13451:SF0 - CROSSOVER JUNCTION ENDONUCLEASE MUS81; Crossover junction endonuclease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGATCTGCTCCTCGTCAGAGTCGGGCT |
| Internal bar code: | CACCAACAGTTGTCGTCTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.8496 |
| LEAP-Seq n confirming: | 1992 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCATGAAAGAGGGTGT |
| Suggested primer 2: | GATGCTGCTCAGAAGAAGGC |