| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177180 |
| Chromosome: | chromosome 2 |
| Location: | 6370033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115400 | (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTCGGCTGCAGTGCCCATGGAGCTCT |
| Internal bar code: | TATTTCGGCTACTCGGGTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.334 |
| LEAP-Seq n confirming: | 1044 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGATACGGCTCCACCTC |
| Suggested primer 2: | CTCGTTAGAGACCAGCCCAG |