| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177194 |
| Chromosome: | chromosome 9 |
| Location: | 1341119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398700 | CFA2,CPLD27 | (1 of 1) 2.1.1.140 - (S)-coclaurine-N-methyltransferase; coclaurine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCGGTACACCGTGCCCCTCTCGTCCCG |
| Internal bar code: | CGGACGGGTACTTACTTTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.6111 |
| LEAP-Seq n confirming: | 5891 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTCGAAACACCGCCAAC |
| Suggested primer 2: | AGCGTAAGAGAGGGGAGAGG |