| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177199 |
| Chromosome: | chromosome 1 |
| Location: | 5453628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038350 | (1 of 39) IPR009003 - Peptidase S1, PA clan | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCCTCTACCTGCTGCACGCCCTCAATC |
| Internal bar code: | CTCGTCGTGGTTTAACGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.0978 |
| LEAP-Seq n confirming: | 7579 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGACCAAAGAGCAGCATA |
| Suggested primer 2: | AAGGTGTCCAATGCAAGGTC |