Insertion junction: LMJ.RY0402.177201_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.177201
Chromosome:chromosome 12
Location:2981267
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g501150 MLT2 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Multi eye protein 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCGTGACCCCGCCTCCGCCTCCACGCTA
Internal bar code:CACGGTAGGTGACTGCAGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:TGACACACCGGCATCATAGT
Suggested primer 2:AGACAGGGAATGTGGACGAC