| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177204 |
| Chromosome: | chromosome 9 |
| Location: | 4306968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394769 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCTGTGGGACAGGCAGTTGGGCCGA |
| Internal bar code: | AGCCCGCCTTAGGTTAAAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 79.9819 |
| LEAP-Seq n confirming: | 3536 |
| LEAP-Seq n nonconfirming: | 885 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACAACACCCACGTTGC |
| Suggested primer 2: | AGTGGAGTGGAGTGGAGTGG |