| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177214 |
| Chromosome: | chromosome 10 |
| Location: | 4685536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453400 | MSC4,MSCL1 | (1 of 1) PF00924//PF13202 - Mechanosensitive ion channel (MS_channel) // EF hand (EF-hand_5); Predicted protein with mechanosensitive ion channel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAACCCCCATCTCCACAACCCTCGCAA |
| Internal bar code: | TTACGAACTGCGTGTTTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 98.4925 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCCTTCTCCAGCGACG |
| Suggested primer 2: | GGCGCTGGCTCTACTACATC |