Insertion junction: LMJ.RY0402.177233_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.177233
Chromosome:chromosome 11
Location:373016
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCGAGCAGTCGTCACACACGGGCAGCT
Internal bar code:CACAGGAGACGGACAATGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:535
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:513
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCAACTGATCCAGACACA
Suggested primer 2:CCTTCGGACTGTCGAATGTT